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HS1BP3 antibody [C2C3], C-term

KO/KD Orthogonal
Anti-HS1BP3 antibody [C2C3], C-term used in Western Blot (WB). GTX107715
Anti-HS1BP3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107715
Anti-HS1BP3 antibody [C2C3], C-term used in Western Blot (WB). GTX107715
Anti-HS1BP3 antibody [C2C3], C-term used in Western Blot (WB). GTX107715

Cat. No. GTX107715

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all HS1BP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HS1BP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240965

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HCLS1 binding protein 3 , ETM2 , HS1-BP3

Background

The protein encoded by this gene shares similarity with mouse Hs1bp3, an Hcls1/Hs1-interacting protein that may be involved in lymphocyte activation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HS1BP3 antibody [C2C3], C-term used in Western Blot (WB). GTX107715

GTX107715 WB Image

HS1BP3 antibody detects HS1BP3 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HS1BP3 antibody (GTX107715) at a dilution of 1:10000.

Anti-HS1BP3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107715

GTX107715 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using HS1BP3(GTX107715) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HS1BP3 antibody [C2C3], C-term used in Western Blot (WB). GTX107715

GTX107715 WB Image

KO/KD Orthogonal

The data was published in the journal Nat Commun in 2016. PMID: 28004827

Anti-HS1BP3 antibody [C2C3], C-term used in Western Blot (WB). GTX107715

GTX107715 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2016. PMID: 28004827

REFERENCE

Application Reference

Holland P et al. Nat Commun 2016; 7 : 13889 HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169