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HSC70 antibody [C1C3]

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150
Anti-HSC70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111150
Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150
Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150
Anti-HSC70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111150
Anti-HSC70 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111150

Cat. No. GTX111150

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Pig
Package
100 μl ($399),
25 μl ($169)
See all HSC70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Raw264.7 , Mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Japanese Medaka, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950525

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

Cellular Localization

Cytoplasm , Melanosome

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150

GTX111150 WB Image

Sample (20 μg of whole cell lysate)
A: Raw264.7
GTX111150 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111150

GTX111150 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70(GTX111150) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150

GTX111150 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111150 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150

GTX111150 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: HeLa
D: HepG2 (GTX27900)
7.5% SDS PAGE
GTX111150 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111150

GTX111150 IHC-P Image

The data was published in the journal PLoS One in 2017. PMID: 28505189

Anti-HSC70 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111150

GTX111150 ICC/IF Image

The data was published in the journal PLoS One in 2017. PMID: 28505189

REFERENCE

Application Reference

Rengstl B et al. PLoS One 2017; 12 (5) : e0177378 Small and big Hodgkin-Reed-Sternberg cells of Hodgkin lymphoma cell lines L-428 and L-1236 lack consistent differences in gene expression profiles and are capable to reconstitute each other.
Application : IHC-P
Reactivity : Human
Yun-Hong Y et al. Mol Cell Proteomics 2011; 10 (10) : M110.007138 A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
Application : WB
Reactivity : Pig
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169