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HSC70 antibody [C1C3]

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150
Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150
Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150
Anti-HSC70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111150

Cat No. GTX111150

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Pig
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , Raw264.7 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950525

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

Cellular Localization

Cytoplasm , Melanosome

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150

GTX111150 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111150 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150

GTX111150 WB Image

Sample (20 μg of whole cell lysate)
A: Raw264.7
GTX111150 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [C1C3] used in Western Blot (WB). GTX111150

GTX111150 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: HeLa
D: HepG2 (GTX27900)
7.5% SDS PAGE
GTX111150 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111150

GTX111150 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70(GTX111150) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

HSC70 antibody [C1C3] Cat No. GTX111150

Rating ( Average 5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 28505189
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 28505189
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169