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HSC70 antibody [HL1788]

Recombinant antibody Overexpression
Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440
Anti-HSC70 antibody [HL1788] used in IHC (Paraffin sections) (IHC-P). GTX637440
Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440
Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440
Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440

Cat. No. GTX637440

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1788

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all HSC70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:30000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Product Note

This antibody is specific for human HSC70 protein, and it does not cross react with human HSPA1A, HSPA1L, HSPA2, HSPA5, HSPA6, and HSPA9 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human HSC70.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

Cellular Localization

Cytoplasm,Melanosome,Cell membrane,Nucleolus

Background

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440

GTX637440 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [HL1788] used in IHC (Paraffin sections) (IHC-P). GTX637440

GTX637440 IHC-P Image

HSC70 antibody [HL1788] detects HSC70 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
HSC70 stained by HSC70 antibody [HL1788] (GTX637440) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440

GTX637440 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440

GTX637440 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [HL1788] used in Western Blot (WB). GTX637440

GTX637440 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody [HL1788] (GTX637440) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations