APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.03-0.1μg/ml |
| 10μg/ml |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of HeLa cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89273-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_006588.1) only.
Predict Reactivity
Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71
Cellular Localization
Cytoplasm , Cell membrane , Nucleolus
Background
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
|
GTX89273 WB Image
WB analysis of HepG2 (A) and NIH-3T3 (B) cell lysate using GTX89273 HSC70 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX89273 ICC/IF Image
ICC/IF analysis of PFA fixed HeLa cells using GTX89273 HSC70 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
|
REFERENCE
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REVIEW
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