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HSC70 antibody, Internal

Anti-HSC70 antibody, Internal used in Western Blot (WB). GTX89273
Anti-HSC70 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89273

Cat No. GTX89273

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
ICC/IF 10μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of HeLa cells.

Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89273-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_006588.1) only.

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

Cellular Localization

Cytoplasm , Cell membrane , Nucleolus

Background

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-HSC70 antibody, Internal used in Western Blot (WB). GTX89273

GTX89273 WB Image

WB analysis of HepG2 (A) and NIH-3T3 (B) cell lysate using GTX89273 HSC70 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

Anti-HSC70 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89273

GTX89273 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX89273 HSC70 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 369