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HSC70 antibody [N2C1], Internal

Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

Cat. No. GTX111069

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HSC70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , A431 , HeLa , 293T , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

Cellular Localization

Cytoplasm , Melanosome

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

GTX111069 WB Image

Hsc70 antibody detects Hsc70 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Hsc70 antibody (GTX111069) dilution: 1:5000

Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069

GTX111069 IHC-P Image

HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX111069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069

GTX111069 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70(GTX111069) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111069

GTX111069 ICC/IF Image

HSC70 antibody detects HSC70 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSC70 protein stained by HSC70 antibody (GTX111069) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

GTX111069 WB Image

Sample (30 μg of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX111069 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069

GTX111069 IHC-P Image

HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX111069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

GTX111069 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HeLa
7.5% SDS PAGE
GTX111069 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Fussi N et al. Cell Death Dis 2018; 9 (7) : 757 Exosomal secretion of α-synuclein as protective mechanism after upstream blockage of macroautophagy.
Application : WB
Reactivity : Human
Soliman M et al. PLoS Pathog 2018; 14 (1) : e1006820 Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.
Application : WB
Reactivity : Human
Luu BE et al. Cell Stress Chaperones 2017; The regulation of heat shock proteins in response to dehydration in Xenopus laevis.
Application : WB
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169