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HSC70 antibody [N2C1], Internal

Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069
Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

Cat No. GTX111069

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all HSC70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A431 , HeLa , Neuro2A , GL261 , C8D30 , 293T , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950526

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

Cellular Localization

Cytoplasm , Melanosome

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069

GTX111069 IHC-P Image

HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX111069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069

GTX111069 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70(GTX111069) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111069

GTX111069 ICC/IF Image

HSC70 antibody detects HSC70 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSC70 protein stained by HSC70 antibody (GTX111069) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HSC70 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111069

GTX111069 IHC-P Image

HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX111069) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

GTX111069 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HeLa
7.5% SDS PAGE
GTX111069 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

GTX111069 WB Image

Sample (30 μg of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX111069 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody [N2C1], Internal used in Western Blot (WB). GTX111069

GTX111069 WB Image

HSC70 antibody detects HSC70 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
HSC70 antibody (GTX111069) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Fussi N et al. Cell Death Dis 2018; 9 (7) : 757 Exosomal secretion of α-synuclein as protective mechanism after upstream blockage of macroautophagy.
Application : WB
Reactivity : Human
Soliman M et al. PLoS Pathog 2018; 14 (1) : e1006820 Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.
Application : WB
Reactivity : Human
Luu BE et al. Cell Stress Chaperones 2017; The regulation of heat shock proteins in response to dehydration in Xenopus laevis.
Application : WB
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169