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HSC70 antibody

Orthogonal
Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144
Anti-HSC70 antibody used in Western Blot (WB). GTX101144
Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144
Anti-HSC70 antibody used in Western Blot (WB). GTX101144
Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144
Anti-HSC70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101144
Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144
Anti-HSC70 antibody used in Western Blot (WB). GTX101144

Cat. No. GTX101144

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all HSC70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Bovine, Chicken, Pig, Japanese Medaka, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HSC70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71

Cellular Localization

Cytoplasm , Melanosome

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144

GTX101144 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HSC70 antibody (GTX101144) at 1:300 dilution.

Anti-HSC70 antibody used in Western Blot (WB). GTX101144

GTX101144 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101144 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144

GTX101144 IHC-P Image

HSC70 antibody detects HSC70 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
HSC70 stained by HSC70 antibody (GTX101144) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15

Anti-HSC70 antibody used in Western Blot (WB). GTX101144

GTX101144 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (GTX101144) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144

GTX101144 IHC-P Image

HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
HSC70 antibody (GTX101144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101144

GTX101144 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HSC70(GTX101144) antibody at 1:200 dilution.

Anti-HSC70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101144

GTX101144 IHC-P Image

HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX101144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSC70 antibody used in Western Blot (WB). GTX101144

GTX101144 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (GTX101144) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Rufino-Ramos D et al. Mol Ther 2023; 31 (7) : 2220-2239 Extracellular communication between brain cells through functional transfer of Cre mRNA mediated by extracellular vesicles.
Rufino-Ramos D et al. Mol Ther 2023; 31 (5) : 1275-1292 Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease/spinocerebellar ataxia type 3.
Rufino-Ramos D et al. bioRxiv 2023; Extracellular communication between brain cells through functional transfer of Cre mRNA.
Application : WB
Reactivity : Human
Salim C et al. J Orofac Orthop 2021; Role of chaperone-assisted selective autophagy (CASA) in mechanical stress protection of periodontal ligament cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169