GeneTex
United States (US)

HSC70 antibody

GTX101144_39568_IFA_19011506_782.jpg
GTX101144_39568_IHC_19011506_302.jpg
GTX101144_39568_IHC_R_19011506_392.jpg
GTX101144_39568_IHC_M_19011506_471.jpg
GTX101144_42648_20161117_WB_19011506_195.jpg
GTX101144_39568_WB_M_brain_19011506_592.jpg

Cat No. GTX101144

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HSC70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family A (Hsp70) Member 8,Hel-33,Hel-S-72P,Hsc54,Hsc70,Hsc71,Hsp71,Hsp73,Hspa10,Lap-1,Lap1,Nip71,Hspa8

Cellular Localization

Cytoplasm , Melanosome

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101144_39568_IFA_19011506_782.jpg

GTX101144 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HSC70(GTX101144) antibody at 1:200 dilution.

GTX101144_39568_IHC_19011506_302.jpg

GTX101144 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSC70(GTX101144) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101144_39568_IHC_R_19011506_392.jpg

GTX101144 IHC-P Image

HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
HSC70 antibody (GTX101144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101144_39568_IHC_M_19011506_471.jpg

GTX101144 IHC-P Image

HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX101144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101144_42648_20161117_WB_19011506_195.jpg

GTX101144 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (GTX101144) diluted at 1:10000.

GTX101144_39568_WB_M_brain_19011506_592.jpg

GTX101144 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101144 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169