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HSD17B3 antibody

Anti-HSD17B3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114480
Anti-HSD17B3 antibody used in Western Blot (WB). GTX114480
Anti-HSD17B3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114480
Anti-HSD17B3 antibody used in Western Blot (WB). GTX114480

Cat. No. GTX114480

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HSD17B3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD17B3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171352

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxysteroid 17-beta dehydrogenase 3 , EDH17B3 , SDR12C2

Background

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSD17B3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114480

GTX114480 ICC/IF Image

HSD17B3 antibody detects HSD17B3 protein at cytoplasm by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSD17B3 protein stained by HSD17B3 antibody (GTX114480) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-HSD17B3 antibody used in Western Blot (WB). GTX114480

GTX114480 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSD17B3 antibody (GTX114480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSD17B3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114480

GTX114480 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSD17B3 antibody(GTX114480) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSD17B3 antibody used in Western Blot (WB). GTX114480

GTX114480 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSD17B3 antibody (GTX114480) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Feng X et al. Mol Cell Endocrinol 2021; 519 : 111039 Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.
Application : ICC/IF
Reactivity : Human
Kobayashi H et al. Exp Anim 2020; (Epub) Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to Wistar rats.
Application : WB
Reactivity : Rat
Zimmer BM et al. Horm Cancer 2016; 7 (4) : 260-71 Loss of exogenous androgen dependence by prostate tumor cells is associated with elevated glucuronidation potential.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169