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HSD17B4 antibody

Anti-HSD17B4 antibody used in Western Blot (WB). GTX103864
Anti-HSD17B4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103864
Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103864
Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103864
Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103864

Cat. No. GTX103864

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all HSD17B4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Rat heart

Predict Reactivity

Mouse, Bovine, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD17B4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxysteroid 17-beta dehydrogenase 4 , DBP , MFE-2 , MPF-2 , PRLTS1 , SDR8C1

Cellular Localization

Peroxisome

Background

The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSD17B4 antibody used in Western Blot (WB). GTX103864

GTX103864 WB Image

HSD17B4 antibody detects HSD17B4 protein by western blot analysis.
A. 20 μg Rat heart lysate/extract
7.5% SDS-PAGE
HSD17B4 antibody (GTX103864) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSD17B4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103864

GTX103864 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using HSD17B4(GTX103864) antibody at 1:500 dilution.

Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103864

GTX103864 IHC-P Image

HSD17B4 antibody detects HSD17B4 protein at mitochondria in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
HSD17B4 antibody (GTX103864) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103864

GTX103864 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using HSD17B4(GTX103864) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103864

GTX103864 IHC-P Image

HSD17B4 antibody detects HSD17B4 protein at mitochondria in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
HSD17B4 antibody (GTX103864) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Frasinyuk MS et al. Org Biomol Chem 2017; 15 (36) : 7623-7629 Developing antineoplastic agents that target peroxisomal enzymes: cytisine-linked isoflavonoids as inhibitors of hydroxysteroid 17-beta-dehydrogenase-4 (HSD17B4).
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169