HSD17B4 antibody

3T3-L1 and differentiated 3T3-L1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSD17B4 antibody (GTX114978) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSD17B4 antibody (GTX114978) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

HSD17B4 antibody detects HSD17B4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSD17B4 protein stained by HSD17B4 antibody (GTX114978) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HSD17B4(GTX114978) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX114978 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX114978 diluted at 1:1000