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HSD17B4 antibody

Orthogonal
Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978
Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978
Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114978
Anti-HSD17B4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114978
Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978
Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978

Cat. No. GTX114978

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HSD17B4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Mouse brain , HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Guinea pig, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human HSD17B4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620181

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxysteroid 17-beta dehydrogenase 4 , DBP , MFE-2 , MPF-2 , PRLTS1 , SDR8C1

Cellular Localization

Peroxisome

Background

The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978

GTX114978 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX114978 diluted at 1:1000

Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978

GTX114978 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX114978 diluted at 1:1000

Anti-HSD17B4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114978

GTX114978 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HSD17B4(GTX114978) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSD17B4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114978

GTX114978 ICC/IF Image

HSD17B4 antibody detects HSD17B4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSD17B4 protein stained by HSD17B4 antibody (GTX114978) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978

GTX114978 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSD17B4 antibody (GTX114978) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HSD17B4 antibody used in Western Blot (WB). GTX114978

GTX114978 WB Image

Orthogonal

3T3-L1 and differentiated 3T3-L1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSD17B4 antibody (GTX114978) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Aline Yammine et al. Cells 2020; 9 (11) Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169