HSD3B2 antibody [N3C3]

Cat. No. GTX102813

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all HSD3B2 products

View product citations for antibody GTX102813 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD3B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950513

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 , HSD3B , HSDB , SDR11E2

Cellular Localization

Endoplasmic reticulum membrane , Mitochondrion membrane

Background

The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3284 HSD3B2
UniProt: P26439 HSD3B2

Research Area

DATA IMAGES

GTX102813 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HSD3B2(GTX102813) antibody at 1:200 dilution.

GTX102813 WB Image

Mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HSD3B2 antibody [N3C3] (GTX102813) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102813 IHC-P Image

HSD3B2 antibody [N3C3] detects HSD3B2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
HSD3B2 stained by HSD3B2 antibody [N3C3] (GTX102813) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min