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HSD3B2 antibody [N3C3]

Anti-HSD3B2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102813
Anti-HSD3B2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102813
Anti-HSD3B2 antibody [N3C3] used in Western Blot (WB). GTX102813
Anti-HSD3B2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102813

Cat No. GTX102813

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD3B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 , HSD3B , HSDB , SDR11E2

Cellular Localization

Endoplasmic reticulum membrane , Mitochondrion membrane

Background

The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HSD3B2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102813

GTX102813 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HSD3B2(GTX102813) antibody at 1:200 dilution.

Anti-HSD3B2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102813

GTX102813 IHC-P Image

HSD3B2 antibody [N3C3] detects HSD3B2 protein at cytoplasm on mouse ovart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovart.
HSD3B2 antibody [N3C3] (GTX102813) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSD3B2 antibody [N3C3] used in Western Blot (WB). GTX102813

GTX102813 WB Image

HSD3B2 antibody [N3C3] detects HSD3B2 protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
10 % SDS-PAGE
HSD3B2 antibody [N3C3] (GTX102813) dilution: 1:500

Anti-HSD3B2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102813

GTX102813 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using HSD3B2(GTX102813) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169