GeneTex
United States (US)

HSD3B2 antibody

GTX102744_WB_19011506_119.jpg
GTX102744_39897_IHC_19011506_289.jpg

Cat No. GTX102744

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4

Predict Reactivity

Cat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD3B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HSD3B antibody, HSDB antibody, SDR11E2 antibody, HSD3B2 antibody, short chain dehydrogenase/reductase family 11E, member 2 antibody, 3-beta-HSD II antibody, 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II antibody, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody, 3 beta-HSD type II antibody, progesterone reductase antibody, delta 5-delta 4-isomerase type II antibody, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II antibody, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase antibody, 3-beta-HSD adrenal and gonadal type antibody, 3-beta-hydroxy-5-ene steroid dehydrogenase antibody, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 antibody

Cellular Localization

Endoplasmic reticulum membrane , Mitochondrion membrane

Background

The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102744_WB_19011506_119.jpg

GTX102744 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
B:MOLT4(GTX27912)
12% SDS PAGE
GTX102744 diluted at 1:500

GTX102744_39897_IHC_19011506_289.jpg

GTX102744 IHC-P Image

HSD3B2 antibody detects HSD3B2 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
HSD3B2 antibody (GTX102744) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

HSD3B2 antibody Cat No. GTX102744

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Jun-2018
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Leydig cells
Fixation : 4% PFA, 4°C, 0.17Hr
Primary Antibody : 1:100, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169