GeneTex

  • Country / Location Selection

United States (US)

HSD3B2 antibody

Anti-HSD3B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102744
Anti-HSD3B2 antibody used in Western Blot (WB). GTX102744
Anti-HSD3B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102744

Cat. No. GTX102744

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HSD3B2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4

Predict Reactivity

Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD3B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950512

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 , HSD3B , HSDB , SDR11E2

Cellular Localization

Endoplasmic reticulum membrane , Mitochondrion membrane

Background

The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSD3B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102744

GTX102744 IHC-P Image

HSD3B2 antibody detects HSD3B2 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
HSD3B2 antibody (GTX102744) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSD3B2 antibody used in Western Blot (WB). GTX102744

GTX102744 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
B:MOLT4(GTX27912)
12% SDS PAGE
GTX102744 diluted at 1:500

Anti-HSD3B2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102744

GTX102744 IHC-P Image

The data was published in the journal Sci Rep in 2019. PMID: 31367001

REFERENCE

Application Reference

Liu SW et al. Sci Rep 2019; 9 (1) : 11143 A Tri-fusion Reporter Mouse Reveals Tissue-Specific FGF1B Promoter Activity in vivo.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

HSD3B2 antibody Cat. No. GTX102744

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-HSD3B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102744
Date : Anonymous submitted on 05-Jun-2018
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Leydig cells
Fixation : 4% PFA, 4°C, 0.17Hr
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169