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HSD3a antibody, N-term

Anti-HSD3a antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89330

Cat. No. GTX89330

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all HSD3a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Liver shows staining of distinct structures in the cytoplasm of hepatocytes.
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89330-PEP).

Product Note

This antibody may cross-react with AKR1C1

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence DPKYQRVELNDGH-C, from the N Terminus of the protein sequence according to NP_001809.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member C4 , 3-alpha-HSD , C11 , CDR , CHDR , DD-4 , DD4 , HAKRA

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HSD3a antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89330

GTX89330 IHC-P Image

IHC-P analysis of human liver using GTX89330 HSD3a antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

Application Reference

Guo SS et al. BMC Cancer 2022; 22 (1) : 880 Prognostic significance of AKR1C4 and the advantage of combining EBV DNA to stratify patients at high risk of locoregional recurrence of nasopharyngeal carcinoma.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399