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HSD3a antibody [N1C2]

Anti-HSD3a antibody [N1C2] used in Western Blot (WB). GTX103613
Anti-HSD3a antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103613
Anti-HSD3a antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103613

Cat No. GTX103613

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD3a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aldo-keto reductase family 1 member C4 , 3-alpha-HSD , C11 , CDR , CHDR , DD-4 , DD4 , HAKRA

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HSD3a antibody [N1C2] used in Western Blot (WB). GTX103613

GTX103613 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
HSD3a antibody
GTX103613 diluted at 1:1000

Anti-HSD3a antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103613

GTX103613 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKR1C4(GTX103613) antibody at 1:100 dilution.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSD3a antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103613

GTX103613 ICC/IF Image

HSD3a antibody [N1C2] detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSD3a protein stained by HSD3a antibody [N1C2] (GTX103613) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
There are currently no references for HSD3a antibody [N1C2] (GTX103613). Be the first to share your publications with this product.
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf