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HSF1 antibody

Anti-HSF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04342
Anti-HSF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04342
Anti-HSF1 antibody used in Western Blot (WB). GTX04342
Anti-HSF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04342
Anti-HSF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04342

Cat. No. GTX04342

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HSF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-2000
ICC/IF 1:50-100
IHC-P 1:50-100
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol (may contain BSA, please contact us for BSA-free format)

Preservative

0.01% Thiomersal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_005517.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock transcription factor 1 , HSTF1

Cellular Localization

Nucleus , Cytoplasm , nucleoplasm , Cytoplasm, perinuclear region , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Background

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-HSF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04342

GTX04342 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX04342 HSF1 antibody.
Antigen retrieval : Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2.
Dilution : 1:100

Anti-HSF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04342

GTX04342 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX04342 HSF1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-HSF1 antibody used in Western Blot (WB). GTX04342

GTX04342 WB Image

WB analysis of various cell lysates using GTX04342 HSF1 antibody.
Dilution : 1:3000
Loading : 25 μg

Anti-HSF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04342

GTX04342 ICC/IF Image

ICC/IF analysis of C6 cells using GTX04342 HSF1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-HSF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04342

GTX04342 ICC/IF Image

ICC/IF analysis of L929 cells using GTX04342 HSF1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399