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HSF1 (phospho Ser307) antibody

Anti-HSF1 (phospho Ser307) antibody used in Western Blot (WB). GTX50239
Anti-HSF1 (phospho Ser307) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50239
Anti-HSF1 (phospho Ser307) antibody used in Western Blot (WB). GTX50239

Cat. No. GTX50239

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all HSF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 307 (P-Q-S(p)-P-R) derived from human HSF1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock transcription factor 1 , HSTF1

Cellular Localization

Nucleus,Cytoplasm,nucleoplasm

Background

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-HSF1 (phospho Ser307) antibody used in Western Blot (WB). GTX50239

GTX50239 WB Image

WB analysis of extracts from HUVEC cells untreated (lane 1) or treated with TNF-a (lane 2) using GTX50239 HSF1 (phospho Ser307) antibody.

Anti-HSF1 (phospho Ser307) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50239

GTX50239 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50239 HSF1 (phospho Ser307) antibody.

Anti-HSF1 (phospho Ser307) antibody used in Western Blot (WB). GTX50239

GTX50239 WB Image

WB analysis of extracts from HeLa cells treated with IFNa or calf intestinal phosphatase (CIP) using GTX50239 HSF1 (phospho Ser307) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399