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HSF1 (phospho Ser307) antibody

Anti-HSF1 (phospho Ser307) antibody used in Western Blot (WB). GTX79052

Cat. No. GTX79052

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all HSF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-Sp-P-R).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11176410

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HSF1,HSTF1,heat shock transcription factor 1

Cellular Localization

Cytoplasm,Nucleus

Background

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-HSF1 (phospho Ser307) antibody used in Western Blot (WB). GTX79052

GTX79052 WB Image

WB analysis of HUVEC cell treated with TNF-a lysate using GTX79052 HSF1 (phospho Ser307) antibody (Lane 3 and 4) and HSF1 antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399