GeneTex
United States (US)

HSH2D antibody, Internal

GTX88192_2502_WB_18121411_630.jpg

Cat No. GTX88192

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88192-PEP).

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HSHVGYTLSYKAQ, from the internal region of the protein sequence according to NP_116244.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hematopoietic Sh2 Domain Containing,Alx,Hsh2,Hsh2D

Background

T-cell activation requires 2 signals: recognition of antigen by the T-cell receptor (see TCR; MIM 186880) and a costimulatory signal provided primarily by CD28 (MIM 186760) in naive T cells. HSH2 is a target of both of these signaling pathways (Greene et al., 2003 [PubMed 12960172]).[supplied by OMIM, Mar 2008]

Database

DATA IMAGES
GTX88192_2502_WB_18121411_630.jpg

GTX88192 WB Image

WB analysis of K562 lysate using GTX88192 HSH2D antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329