APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Guinea pig, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human HSL around the phosphorylation site of Ser853.
Purification
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lipase E, hormone sensitive type , AOMS4 , FPLD6 , HSL , LHS
Cellular Localization
Cell membrane , Cytoplasm, cytosol
Background
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX01573 IHC-P Image
IHC-P analysis of human thyroid cancer tissue using GTX01573 HSL (phospho Ser853) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX01573 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX01573 HSL (phospho Ser853) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX01573 WB Image
WB analysis of 3T3-L1 cell treated with isoproterenol lysate using GTX01573 HSL (phospho Ser853) antibody.
Lane 1 : Primary antibody pre-treated with phospho-blocking peptide
Lane2 : Primary antibody pre-treated with non-phospho-blocking peptide
Lane 3 : untreated sample
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REFERENCE
There are currently no references for HSL (phospho Ser853) antibody (GTX01573). Be the first to share your publications with this product.
REVIEW
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