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HSP105 antibody [J1G12]

Anti-HSP105 antibody [J1G12] used in Western Blot (WB). GTX50045

Cat. No. GTX50045

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 J1G12

Isotype

 IgG2b

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all HSP105 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Hsp105 (1-858aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family H (Hsp110) member 1 , HSP105 , HSP105A , HSP105B , NY-CO-25

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]

Database

Research Area

DATA IMAGES

Anti-HSP105 antibody [J1G12] used in Western Blot (WB). GTX50045

GTX50045 WB Image

Cell lysates of HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp105 alpha (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399