GeneTex
United States (US)

HSP22 antibody [C2C3], C-term

GTX104815_IFA_19011506_485.jpg
GTX104815_39946_IHC_19011506_511.jpg
GTX104815_WB_19011506_465.jpg

Cat No. GTX104815

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HSP22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family B (Small) Member 8,Cmt2L,Dhmn2,E2Ig1,H11,Hmn2,Hmn2A,Hsp22,Hspb8

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq]

Database

DATA IMAGES
GTX104815_IFA_19011506_485.jpg

GTX104815 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HSPB8(GTX104815) antibody at 1:200 dilution.

GTX104815_39946_IHC_19011506_511.jpg

GTX104815 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using HSP22(GTX104815) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104815_WB_19011506_465.jpg

GTX104815 WB Image

Sample(30 ug whole cell lysate)
A:293T whole cell lysate
B:A431(GTX27909)
12% SDS PAGE
GTX104815 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169