APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
1:100 |
1:100 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% glycerol
Preservative
0.09% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human HSP22
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family B (small) member 8 , CMT2L , DHMN2 , E2IG1 , H11 , HMN2 , HMN2A , HSP22
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04663 WB Image
WB analysis of rat skeletal muscle tissue lysate using GTX04663 HSP22 antibody. Loading : 15 μg Dilution : 1:1000
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GTX04663 IHC-P Image
IHC-P analysis of mouse backskin tissue using GTX04663 HSP22 antibody. Localization : Epidermis positive, dermal staining. Fixation: Bouin's Fixative Solution. Dilution : 1:100
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GTX04663 ICC/IF Image
ICC/IF analysis of cold 100% methanol-fixed HaCaT cells using GTX04663 HSP22 antibody. Dilution : 1:100
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REFERENCE
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REVIEW
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