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HSP22 antibody

Anti-HSP22 antibody used in Western Blot (WB). GTX04663
Anti-HSP22 antibody used in IHC (Paraffin sections) (IHC-P). GTX04663
Anti-HSP22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04663

Cat. No. GTX04663

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HSP22 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol

Preservative

0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HSP22

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 8 , CMT2L , DHMN2 , E2IG1 , H11 , HMN2 , HMN2A , HSP22

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HSP22 antibody used in Western Blot (WB). GTX04663

GTX04663 WB Image

WB analysis of rat skeletal muscle tissue lysate using GTX04663 HSP22 antibody.
Loading : 15 μg
Dilution : 1:1000

Anti-HSP22 antibody used in IHC (Paraffin sections) (IHC-P). GTX04663

GTX04663 IHC-P Image

IHC-P analysis of mouse backskin tissue using GTX04663 HSP22 antibody.
Localization : Epidermis positive, dermal staining.
Fixation: Bouin's Fixative Solution.
Dilution : 1:100

Anti-HSP22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04663

GTX04663 ICC/IF Image

ICC/IF analysis of cold 100% methanol-fixed HaCaT cells using GTX04663 HSP22 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399