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HSP25 antibody

Anti-HSP25 antibody used in Western Blot (WB). GTX12351

Cat. No. GTX12351

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Mouse, Rat, Hamster, Guinea pig, Pig
Package
50 μl ($399)
See all HSP25 products

APPLICATION

Application Note

For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Hsp25 Protein and NSP-510

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse Hsp25

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_371582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Background

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the ""small heat shock proteins"" is comprised of a diverse group of proteins from ~15 to >30kDa, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated stress protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores. The expression of a 24kDa protein was shown to correlate with steroid hormone receptors in cell lines, organs , and tumors. The 24kDa estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that the levels of Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the drosophila Hsp20 genes.

Research Area

DATA IMAGES

Anti-HSP25 antibody used in Western Blot (WB). GTX12351

GTX12351 WB Image

Western Blot analysis of HSP25, pAb.
Lane 1: MW Marker
Lane 2: PC-12 Cell Lysate (Heat Shock)
Lane 3: 3T3 Cell Lysate (Heat Shock)
Lane 4: HSP25 (mouse), (recombinant) (GTX12351)

REFERENCE

Application Reference

Kim MS et al. Diabetes 2008; 57 (1) : 64-76 Acute diabetes moderates trafficking of cardiac lipoprotein lipase through p38 mitogen-activated protein kinase-dependent actin cytoskeleton organization.
Application : WB
Reactivity : Rat
Kewalramani G et al. Am J Physiol Endocrinol Metab 2008; 295 (1) : E137-47 Acute dexamethasone-induced increase in cardiac lipoprotein lipase requires activation of both Akt and stress kinases.
Application : WB
Reactivity : Rat
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Package List Price ($)
$ 399