APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:2000 |
| Assay dependent |
| 1:50-1:100 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone 2A5 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp29 protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family B (small) member 1 , CMT2F , HELS102 , HMN2B , HS.76067 , HSP27 , HSP28 , HSPB1 , Hsp25 , SRP27
Cellular Localization
Cytoplasm,Nucleus,Cytoskeleton,Spindle
Background
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550](/templates/default/js/lightbox/images/loading.gif "Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550")
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GTX57550 FACS Image
FACS analysis of HeLa cells using GTX57550 HSP27 antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg
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GTX57550 IHC-P Image
IHC-P analysis of human colon cancer using GTX57550 HSP27 antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:50
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GTX57550 WB Image
WB analysis of various samples using GTX57550 HSP27 antibody.
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57550 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57550 HSP27 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57550 WB Image
WB analysis of various samples using GTX57550 HSP27 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
|
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GTX57550 FACS Image
FACS analysis of A549 cells using GTX57550 HSP27 antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg
|
REFERENCE
There are currently no references for HSP27 antibody [2A5] (GTX57550). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP27 antibody [2A5] (GTX57550). Be the first to share your experience with this product.
![Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550](/upload/website/prouct_img/small/GTX57550/GTX57550_20200204_FC2_w_23061123_314.webp "Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550")
![Anti-HSP27 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX57550](/upload/website/prouct_img/small/GTX57550/GTX57550_20200204_IHC_w_23061123_131.webp "Anti-HSP27 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX57550")
![Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550](/upload/website/prouct_img/small/GTX57550/GTX57550_20200204_WB2_w_23061123_423.webp "Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550")
![Anti-HSP27 antibody [2A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57550](/upload/website/prouct_img/small/GTX57550/GTX57550_20200204_IF_w_23061123_330.webp "Anti-HSP27 antibody [2A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57550")
![Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550](/upload/website/prouct_img/small/GTX57550/GTX57550_20200204_WB1_w_23061123_823.webp "Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550")
![Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550](/upload/website/prouct_img/small/GTX57550/GTX57550_20200204_FC1_w_23061123_913.webp "Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550")
![Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550](/upload/website/prouct_img/normal/GTX57550/GTX57550_20200204_FC2_w_23061123_314.webp "Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550")
![Anti-HSP27 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX57550](/upload/website/prouct_img/normal/GTX57550/GTX57550_20200204_IHC_w_23061123_131.webp "Anti-HSP27 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX57550")
![Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550](/upload/website/prouct_img/normal/GTX57550/GTX57550_20200204_WB2_w_23061123_423.webp "Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550")
![Anti-HSP27 antibody [2A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57550](/upload/website/prouct_img/normal/GTX57550/GTX57550_20200204_IF_w_23061123_330.webp "Anti-HSP27 antibody [2A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57550")
![Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550](/upload/website/prouct_img/normal/GTX57550/GTX57550_20200204_WB1_w_23061123_823.webp "Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550")
![Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550](/upload/website/prouct_img/normal/GTX57550/GTX57550_20200204_FC1_w_23061123_913.webp "Anti-HSP27 antibody [2A5] used in Flow cytometry (FACS). GTX57550")