HSP27 antibody [2A5]

Cat. No. GTX57550
Cat. No.  GTX57550
Anti-HSP27 antibody [2A5] used in Flow cytometry (FCM). GTX57550
GTX57550 FCM Image

FACS analysis of A549 cells using GTX57550 HSP27 antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg

1 / 6
Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550
GTX57550 WB Image

WB analysis of various samples using GTX57550 HSP27 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

2 / 6
Anti-HSP27 antibody [2A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57550
GTX57550 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57550 HSP27 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

3 / 6
Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550
GTX57550 WB Image

WB analysis of various samples using GTX57550 HSP27 antibody.
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

4 / 6
Anti-HSP27 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX57550
GTX57550 IHC-P Image

IHC-P analysis of human colon cancer using GTX57550 HSP27 antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:50

5 / 6
Anti-HSP27 antibody [2A5] used in Flow cytometry (FCM). GTX57550
GTX57550 FCM Image

FACS analysis of HeLa cells using GTX57550 HSP27 antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg

6 / 6
Anti-HSP27 antibody [2A5] used in Flow cytometry (FCM). GTX57550
Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550
Anti-HSP27 antibody [2A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57550
Anti-HSP27 antibody [2A5] used in Western Blot (WB). GTX57550
Anti-HSP27 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX57550
Anti-HSP27 antibody [2A5] used in Flow cytometry (FCM). GTX57550
This HSP27 antibody [2A5] (GTX57550) is a mouse monoclonal antibody validated for detection of HSP27 by WB, ICC/IF, IHC-P, and FCM. Reactivity has been shown in Human.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    2A5
  • Isotype
    IgG1
  • Applications
    WB ICC/IF IHC-P FCM
  • Reactivity
    Human
See all HSP27 products

Ask