APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human HSP27 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
![Anti-HSP27 antibody [5D7] used in Western Blot (WB). GTX60444](/templates/default/js/lightbox/images/loading.gif "Anti-HSP27 antibody [5D7] used in Western Blot (WB). GTX60444")
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GTX60444 WB Image
WB analysis of HeLa (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate using GTX60444 HSP27 antibody [5D7].
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GTX60444 ELISA Image
ELISA analysis of antigen using GTX60444 HSP27 antibody [5D7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60444 FACS Image
FACS analysis of HepG2 cells using GTX60444 HSP27 antibody [5D7].
Green : HSP27
Purple : negative control
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GTX60444 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60444 HSP27 antibody [5D7].
Green : HSP27
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60444 IHC-P Image
IHC-P analysis of breast cancer tissue (left) and cardiac muscle tissue (right) using GTX60444 HSP27 antibody [5D7].
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GTX60444 IHC-P Image
IHC-P analysis of human brain tissue (left) and esophageal cancer tissue (right) using GTX60444 HSP27 antibody [5D7].
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REFERENCE
There are currently no references for HSP27 antibody [5D7] (GTX60444). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP27 antibody [5D7] (GTX60444). Be the first to share your experience with this product.
![Anti-HSP27 antibody [5D7] used in Western Blot (WB). GTX60444](/upload/website/prouct_img/small/GTX60444/GTX60444_20170912_WB_w_23061123_504.webp "Anti-HSP27 antibody [5D7] used in Western Blot (WB). GTX60444")
![Anti-HSP27 antibody [5D7] used in ELISA (ELISA). GTX60444](/upload/website/prouct_img/small/GTX60444/GTX60444_20170912_ELISA_w_23061123_806.webp "Anti-HSP27 antibody [5D7] used in ELISA (ELISA). GTX60444")
![Anti-HSP27 antibody [5D7] used in Flow cytometry (FACS). GTX60444](/upload/website/prouct_img/small/GTX60444/GTX60444_20170912_FACS_w_23061123_211.webp "Anti-HSP27 antibody [5D7] used in Flow cytometry (FACS). GTX60444")
![Anti-HSP27 antibody [5D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60444](/upload/website/prouct_img/small/GTX60444/GTX60444_20170912_ICCIF_w_23061123_897.webp "Anti-HSP27 antibody [5D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60444")
![Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444](/upload/website/prouct_img/small/GTX60444/GTX60444_20170912_IHC-P_1_w_23061123_948.webp "Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444")
![Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444](/upload/website/prouct_img/small/GTX60444/GTX60444_20170912_IHC-P_w_23061123_140.webp "Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444")
![Anti-HSP27 antibody [5D7] used in Western Blot (WB). GTX60444](/upload/website/prouct_img/normal/GTX60444/GTX60444_20170912_WB_w_23061123_504.webp "Anti-HSP27 antibody [5D7] used in Western Blot (WB). GTX60444")
![Anti-HSP27 antibody [5D7] used in ELISA (ELISA). GTX60444](/upload/website/prouct_img/normal/GTX60444/GTX60444_20170912_ELISA_w_23061123_806.webp "Anti-HSP27 antibody [5D7] used in ELISA (ELISA). GTX60444")
![Anti-HSP27 antibody [5D7] used in Flow cytometry (FACS). GTX60444](/upload/website/prouct_img/normal/GTX60444/GTX60444_20170912_FACS_w_23061123_211.webp "Anti-HSP27 antibody [5D7] used in Flow cytometry (FACS). GTX60444")
![Anti-HSP27 antibody [5D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60444](/upload/website/prouct_img/normal/GTX60444/GTX60444_20170912_ICCIF_w_23061123_897.webp "Anti-HSP27 antibody [5D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60444")
![Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444](/upload/website/prouct_img/normal/GTX60444/GTX60444_20170912_IHC-P_1_w_23061123_948.webp "Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444")
![Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444](/upload/website/prouct_img/normal/GTX60444/GTX60444_20170912_IHC-P_w_23061123_140.webp "Anti-HSP27 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX60444")