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HSP27 antibody [G3.1]

Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790
Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790
Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX22790
Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790
Anti-HSP27 antibody [G3.1] used in Immunoprecipitation (IP). GTX22790
Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX22790
Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790
Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX22790
Anti-HSP27 antibody [G3.1] used in Western Blot (WB). GTX22790
Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790

Cat. No. GTX22790

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 G3.1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA, IHC, IM

Reactivity

 Human, Mouse, Rat, Dog, Primate
Package
100 μl ($359)
See all HSP27/Heat Shock Protein 27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:500
IHC-P 1:500
IP 2 μl
ELISA Assay dependent
IHC Assay dependent
IM Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Partially purified human HSP27.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790

GTX22790 ICC/IF Image

ICC/IF analysis of HeLa cells and negative control NIH3T3 cells using GTX22790 HSP27 antibody [G3.1].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790

GTX22790 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX22790

GTX22790 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22790 HSP27 antibody [G3.1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:500

Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790

GTX22790 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-HSP27 antibody [G3.1] used in Immunoprecipitation (IP). GTX22790

GTX22790 IP Image

IP analysis of HeLa cell lysates using GTX22790 HSP27 antibody [G3.1].
GTX22790 was used as IP and IB antibody
IP reaction : 2μg antibody / 500μg lysate
WB antibody dilution : 1:1000

Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX22790

GTX22790 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22790 HSP27 antibody [G3.1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:500

Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790

GTX22790 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX22790

GTX22790 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22790 HSP27 antibody [G3.1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-HSP27 antibody [G3.1] used in Western Blot (WB). GTX22790

GTX22790 WB Image

WB analysis of 50μg of the indicated whole cell lysates using GTX22790 HSP27 antibody [G3.1].
Dilution : 1:1000

Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22790

GTX22790 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 359