HSP27 antibody [G3.1]

ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

WB analysis of 50μg of the indicated whole cell lysates using GTX22790 HSP27 antibody [G3.1].
Dilution : 1:1000

IHC-P analysis of human colon carcinoma tissue using GTX22790 HSP27 antibody [G3.1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

ICC/IF analysis of C6 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

IHC-P analysis of human breast carcinoma tissue using GTX22790 HSP27 antibody [G3.1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:500

IP analysis of HeLa cell lysates using GTX22790 HSP27 antibody [G3.1].
GTX22790 was used as IP and IB antibody
IP reaction : 2μg antibody / 500μg lysate
WB antibody dilution : 1:1000

ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

IHC-P analysis of human tonsil tissue using GTX22790 HSP27 antibody [G3.1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:500

ICC/IF analysis of U251 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of HeLa cells and negative control NIH3T3 cells using GTX22790 HSP27 antibody [G3.1].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature