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HSP27 antibody [G3.1]

Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784
Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784

Cat No. GTX34784

Host Mouse
Clonality Monoclonal
Clone Name G3.1
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Sheep, Chicken, Monkey, Chimpanzee
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25-0.5ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family B (Small) Member 1,Cmt2F,Hel-S-102,Hmn2B,Hs.76067,Hsp27,Hsp28,Hsp25,Srp27,Hspb1

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784

GTX34784 IHC-P Image

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)

Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784

GTX34784 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)

Package List Price ($)
$ 329