APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.25-0.5μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Partially Purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CMT2F , HELS102 , HMN2B , HS.76067 , HSP27 , HSP28 , HSPB1 , Hsp25 , SRP27 , heat shock protein family B (small) member 1
Cellular Localization
Cytoplasm,Nucleus,Cytoskeleton,Spindle
Background
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34784](/templates/default/js/lightbox/images/loading.gif "Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34784")
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GTX34784 ICC/IF Image
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34784 HSP27 antibody [G3.1].
Green : Primary antibody
Red : nucleus
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GTX34784 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX34784 HSP27 antibody [G3.1].
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GTX34784 FACS Image
FACS analysis of PFA-fixed MCF-7 cells using GTX34784 HSP27 antibody [G3.1].
Blue : Primary antibody
Red : Isotype control
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GTX34784 WB Image
WB analysis of HeLa cell lysate using GTX34784 HSP27 antibody [G3.1].
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GTX34784 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX34784 HSP27 antibody [G3.1].
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REFERENCE
There are currently no references for HSP27 antibody [G3.1] (GTX34784). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP27 antibody [G3.1] (GTX34784). Be the first to share your experience with this product.
![Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34784](/upload/website/prouct_img/small/GTX34784/GTX34784_20200115_ICC-IF_1740_w_23060801_885.webp "Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34784")
![Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784](/upload/website/prouct_img/small/GTX34784/GTX34784_20200115_IHC-P_364_w_23060801_100.webp "Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784")
![Anti-HSP27 antibody [G3.1] used in Flow cytometry (FACS). GTX34784](/upload/website/prouct_img/small/GTX34784/GTX34784_20200115_FACS_1719_w_23060801_666.webp "Anti-HSP27 antibody [G3.1] used in Flow cytometry (FACS). GTX34784")
![Anti-HSP27 antibody [G3.1] used in Western Blot (WB). GTX34784](/upload/website/prouct_img/small/GTX34784/GTX34784_20200115_WB_118_w_23060801_603.webp "Anti-HSP27 antibody [G3.1] used in Western Blot (WB). GTX34784")
![Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784](/upload/website/prouct_img/small/GTX34784/GTX34784_20200115_IHC-P_1034_w_23060801_933.webp "Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784")
![Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34784](/upload/website/prouct_img/normal/GTX34784/GTX34784_20200115_ICC-IF_1740_w_23060801_885.webp "Anti-HSP27 antibody [G3.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34784")
![Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784](/upload/website/prouct_img/normal/GTX34784/GTX34784_20200115_IHC-P_364_w_23060801_100.webp "Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784")
![Anti-HSP27 antibody [G3.1] used in Flow cytometry (FACS). GTX34784](/upload/website/prouct_img/normal/GTX34784/GTX34784_20200115_FACS_1719_w_23060801_666.webp "Anti-HSP27 antibody [G3.1] used in Flow cytometry (FACS). GTX34784")
![Anti-HSP27 antibody [G3.1] used in Western Blot (WB). GTX34784](/upload/website/prouct_img/normal/GTX34784/GTX34784_20200115_WB_118_w_23060801_603.webp "Anti-HSP27 antibody [G3.1] used in Western Blot (WB). GTX34784")
![Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784](/upload/website/prouct_img/normal/GTX34784/GTX34784_20200115_IHC-P_1034_w_23060801_933.webp "Anti-HSP27 antibody [G3.1] used in IHC (Paraffin sections) (IHC-P). GTX34784")