*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27
Cytoplasm , Nucleus , Spindle
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]
GTX634459 ICC/IF Image
HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10μm.
GTX634459 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody [GT6310] (GTX634459) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX634459 IHC-P Image
HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX634459 IHC-P Image
HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min