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HSP27 antibody [GT6310]

Anti-HSP27 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634459
Anti-HSP27 antibody [GT6310] used in Western Blot (WB). GTX634459
Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459
Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459

Cat No. GTX634459

Host

Mouse

Clonality

Monoclonal

Clone Name

GT6310

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888455

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm , Nucleus , Spindle

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSP27 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634459

GTX634459 ICC/IF Image

HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-HSP27 antibody [GT6310] used in Western Blot (WB). GTX634459

GTX634459 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody [GT6310] (GTX634459) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459

GTX634459 IHC-P Image

HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459

GTX634459 IHC-P Image

HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for HSP27 antibody [GT6310] (GTX634459). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HSP27 antibody [GT6310] (GTX634459). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169