APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2
Predict Reactivity
Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888455
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27
Cellular Localization
Cytoplasm , Nucleus , Spindle
Background
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459](/templates/default/js/lightbox/images/loading.gif "Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459")
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GTX634459 IHC-P Image
HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634459 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody [GT6310] (GTX634459) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634459 IHC-P Image
HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634459 ICC/IF Image
HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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REFERENCE
There are currently no references for HSP27 antibody [GT6310] (GTX634459). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP27 antibody [GT6310] (GTX634459). Be the first to share your experience with this product.
![Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459](/upload/website/prouct_img/small/GTX634459/GTX634459_43094_20190719_IHC-P_R_w_23061202_806.webp "Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459")
![Anti-HSP27 antibody [GT6310] used in Western Blot (WB). GTX634459](/upload/website/prouct_img/small/GTX634459/GTX634459_43094_20180119_WB_w_23061202_861.webp "Anti-HSP27 antibody [GT6310] used in Western Blot (WB). GTX634459")
![Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459](/upload/website/prouct_img/small/GTX634459/GTX634459_43094_20190719_IHC-P_M_w_23061202_300.webp "Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459")
![Anti-HSP27 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634459](/upload/website/prouct_img/small/GTX634459/GTX634459_43094_20180307_ICC_IF_w_23061202_453.webp "Anti-HSP27 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634459")
![Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459](/upload/website/prouct_img/normal/GTX634459/GTX634459_43094_20190719_IHC-P_R_w_23061202_806.webp "Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459")
![Anti-HSP27 antibody [GT6310] used in Western Blot (WB). GTX634459](/upload/website/prouct_img/normal/GTX634459/GTX634459_43094_20180119_WB_w_23061202_861.webp "Anti-HSP27 antibody [GT6310] used in Western Blot (WB). GTX634459")
![Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459](/upload/website/prouct_img/normal/GTX634459/GTX634459_43094_20190719_IHC-P_M_w_23061202_300.webp "Anti-HSP27 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX634459")
![Anti-HSP27 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634459](/upload/website/prouct_img/normal/GTX634459/GTX634459_43094_20180307_ICC_IF_w_23061202_453.webp "Anti-HSP27 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634459")