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HSP27 antibody [SPM252]

Anti-HSP27 antibody [SPM252] used in Western Blot (WB). GTX34782
Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782
Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782
Anti-HSP27 antibody [SPM252] used in Flow cytometry (FACS). GTX34782
Anti-HSP27 antibody [SPM252] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34782

Cat No. GTX34782

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM252

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Sheep, Chicken, Chimpanzee
Package
100 μg ($339)
See all HSP27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Partially Purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CMT2F , HELS102 , HMN2B , HS.76067 , HSP27 , HSP28 , HSPB1 , Hsp25 , SRP27 , heat shock protein family B (small) member 1

Cellular Localization

Cytoplasm,Nucleus,Cytoskeleton,Spindle

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-HSP27 antibody [SPM252] used in Western Blot (WB). GTX34782

GTX34782 WB Image

WB analysis of HeLa cell lysate using GTX34782 HSP27 antibody [SPM252].

Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782

GTX34782 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34782 HSP27 antibody [SPM252].

Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782

GTX34782 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34782 HSP27 antibody [SPM252].

Anti-HSP27 antibody [SPM252] used in Flow cytometry (FACS). GTX34782

GTX34782 FACS Image

FACS analysis of PFA-fixed MCF-7 cells using GTX34782 HSP27 antibody [SPM252].
Blue : Primary antibody
Red : Isotype control

Anti-HSP27 antibody [SPM252] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34782

GTX34782 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34782 HSP27 antibody [SPM252].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339