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HSP27 antibody [SPM252]

Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782
Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782

Cat No. GTX34782

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM252

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Sheep, Chicken, Chimpanzee
Package
100 μg ($429)
See all HSP27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25-0.5ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Specificity/Sensitivity

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced �p29� and �24K� protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Immunogen

Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Heat Shock Protein Family B (Small) Member 1 , Cmt2F , Hel-S-102 , Hmn2B , Hs.76067 , Hsp27 , Hsp28 , Hsp25 , Srp27 , Hspb1

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782

GTX34782 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (SPM252)

Anti-HSP27 antibody [SPM252] used in IHC (Paraffin sections) (IHC-P). GTX34782

GTX34782 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (SPM252)

REFERENCE

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REVIEW

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Package List Price ($)
$ 429