GeneTex
United States (US)

HSP27 antibody

KO/KD
GTX101145_41710_20160519_WB_shRNA_watermark_19011506_734.jpg
GTX101145_41829_20170406_WB_KO_watermark_19011506_148.jpg
GTX101145_41710_20160125_IHC-P_19011506_767.jpg
GTX101145_41829_20160630_WB_19011506_955.jpg
GTX101145_39533_WB_M_liver_19011506_231.jpg
GTX101145_41710_20150514_IP_19011506_325.jpg
GTX101145_41710_20150410_IFA_19011506_289.jpg

Cat No. GTX101145

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HT-29 , Mouse liver

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family B (Small) Member 1,Cmt2F,Hel-S-102,Hmn2B,Hs.76067,Hsp27,Hsp28,Hsp25,Srp27,Hspb1

Cellular Localization

Cytoplasm , Nucleus , Spindle

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101145_41710_20160519_WB_shRNA_watermark_19011506_734.jpg

GTX101145 WB Image

KO/KD

Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101145_41829_20170406_WB_KO_watermark_19011506_148.jpg

GTX101145 WB Image

KO/KD

Wild-type (WT) and HSP27 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101145_41710_20160125_IHC-P_19011506_767.jpg

GTX101145 IHC-P Image

HSP27 antibody detects HSP27 protein at cytoplasm in human lung papillary carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary carcinoma.
HSP27 antibody (GTX101145) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101145_41829_20160630_WB_19011506_955.jpg

GTX101145 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101145_39533_WB_M_liver_19011506_231.jpg

GTX101145 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX101145 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101145_41710_20150514_IP_19011506_325.jpg

GTX101145 IP Image

Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μg of HSP27 antibody (GTX101145).
Western blot analysis was performed using HSP27 antibody (GTX101145).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101145_41710_20150410_IFA_19011506_289.jpg

GTX101145 ICC/IF Image

HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP27 protein stained by HSP27 antibody (GTX101145) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

HSP27 antibody Cat No. GTX101145

Rating ( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Apr-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Amount used : 50 μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Lane Description : Lane 1: shLacZ (virus infection to silence LacZ gene), Lane 2: shHSP27 (virus infection to silence HSP27 gene)
Date : Anonymous submitted on 25-Sep-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Mesenchymal stem cells
Blocking : 3% BSA, 25°C, 2Hr
Fixation : 4% paraformadehyde
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:100, 4°C, overnightHr
Date : Anonymous submitted on 25-Sep-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : 3A6 cells
Amount used : 54μg
Blocking : 3% BSA, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 24-Oct-2012
Score :
Application Tested : FACS
Sample Species : Hu
Sample : HT29 cells
Fixation : 0.5% skim milk
Primary Antibody : 1:60, 37°C, 1Hr
Figure Note : Blue : Cell alone + goat anti-rabbit-FITC, Red : Hsp27 antibody + goat anti-rabbit-FITC, Green : Unrelated antibody
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169