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HSP27 antibody

Anti-HSP27 antibody used in Western Blot (WB). GTX112964
Anti-HSP27 antibody used in Western Blot (WB). GTX112964
Anti-HSP27 antibody used in Immunoprecipitation (IP). GTX112964
Anti-HSP27 antibody used in Western Blot (WB). GTX112964
Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX112964
Anti-HSP27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112964

Cat No. GTX112964

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HT-29

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172258

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm , Nucleus , Spindle

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSP27 antibody used in Western Blot (WB). GTX112964

GTX112964 WB Image

Wild-type (WT) and HSP27 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX112964) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP27 antibody used in Western Blot (WB). GTX112964

GTX112964 WB Image

Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX112964) diluted at 1:5000.

Anti-HSP27 antibody used in Immunoprecipitation (IP). GTX112964

GTX112964 IP Image

HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 μg of HSP27 (GTX112964) 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody (GTX112964) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HSP27 antibody used in Western Blot (WB). GTX112964

GTX112964 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
12% SDS PAGE
GTX112964 diluted at 1:1000

Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX112964

GTX112964 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSP27(GTX112964) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112964

GTX112964 ICC/IF Image

HSP27 antibody detects HSP27 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP27 protein stained by HSP27 antibody (GTX112964) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169