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HSP27 antibody

KO/KD
Anti-HSP27 antibody used in Western Blot (WB). GTX35243
Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243
Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243
Anti-HSP27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35243
Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243
Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243
Anti-HSP27 antibody used in Western Blot (WB). GTX35243

Cat. No. GTX35243

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HSP27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human HSPB1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-HSP27 antibody used in Western Blot (WB). GTX35243

GTX35243 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35243 HSP27 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243

GTX35243 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX35243 HSP27 antibody.
Dilution : 1:100

Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243

GTX35243 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX35243 HSP27 antibody.
Dilution : 1:100

Anti-HSP27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35243

GTX35243 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX35243 HSP27 antibody.
Blue : DAPI
Dilution : 1:100

Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243

GTX35243 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX35243 HSP27 antibody.
Dilution : 1:100

Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX35243

GTX35243 IHC-P Image

IHC-P analysis of rat brain tissue using GTX35243 HSP27 antibody.
Dilution : 1:100

Anti-HSP27 antibody used in Western Blot (WB). GTX35243

GTX35243 WB Image

WB analysis of HeLa cell lysate using GTX35243 HSP27 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399