ICC/IF analysis of HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes

ICC/IF analysis of HeLa cells treated with 25ug of Anisomycin for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

WB analysis of 50 ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody.
Dilution : 1:500

IP analysis of HeLa cells treated with 10uM Anisomysin for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody.
IP reaction : 3μg antibody / 500μg lysate
Dilution : 1:500