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HSP27 (phospho Ser15) antibody

Orthogonal
Anti-HSP27 (phospho Ser15) antibody used in Immunoprecipitation (IP). GTX25581
Anti-HSP27 (phospho Ser15) antibody used in Western Blot (WB). GTX25581
Anti-HSP27 (phospho Ser15) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25581
Anti-HSP27 (phospho Ser15) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25581

Cat. No. GTX25581

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, IHC

Reactivity

 Human, Rat, Rabbit
Package
100 μg ($399)
See all HSP27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:1000
ICC/IF 1:50 - 1:200
FACS 3-5 μg/10⁶ cells
IP 3 μg
IHC 1:50 - 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide corresponding to the residues L(10) L R G P (pS) W D P F R C(21) of human HSP27.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-HSP27 (phospho Ser15) antibody used in Immunoprecipitation (IP). GTX25581

GTX25581 IP Image

IP analysis of HeLa cells treated with 10uM Anisomysin for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody.
IP reaction : 3μg antibody / 500μg lysate
Dilution : 1:500

Anti-HSP27 (phospho Ser15) antibody used in Western Blot (WB). GTX25581

GTX25581 WB Image

Orthogonal

WB analysis of 50 ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody.
Dilution : 1:500

Anti-HSP27 (phospho Ser15) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25581

GTX25581 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells treated with 25ug of Anisomycin for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-HSP27 (phospho Ser15) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25581

GTX25581 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes using GTX25581 HSP27 (phospho Ser15) antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399