*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:1000
|1:100 - 1:200
Not tested in other applications.
0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of HSP27. The exact sequence is proprietary.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Heat Shock Protein Family B (Small) Member 1,Cmt2F,Hel-S-102,Hmn2B,Hs.76067,Hsp27,Hsp28,Hsp25,Srp27,Hspb1
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
GTX32195 WB Image
WB analysis of Anisomycin-treated HeLa (A), MCF7 (B) whole cell lysates using GTX32195 HSP27 (phospho Ser15) antibody.
GTX32195 IHC-P Image
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32195 HSP27 (phospho Ser15) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)