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HSP27 (phospho Ser15) antibody

Anti-HSP27 (phospho Ser15) antibody used in Western Blot (WB). GTX32195
Anti-HSP27 (phospho Ser15) antibody used in IHC (Paraffin sections) (IHC-P). GTX32195

Cat No. GTX32195

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of HSP27. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
Anti-HSP27 (phospho Ser15) antibody used in Western Blot (WB). GTX32195

GTX32195 WB Image

WB analysis of Anisomycin-treated HeLa (A), MCF7 (B) whole cell lysates using GTX32195 HSP27 (phospho Ser15) antibody.

Anti-HSP27 (phospho Ser15) antibody used in IHC (Paraffin sections) (IHC-P). GTX32195

GTX32195 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32195 HSP27 (phospho Ser15) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE
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REVIEW
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Package List Price ($)
$ 319