GeneTex
United States (US)

HSP27 (phospho Ser15) antibody

GTX38674_20190129_ICCIF_19012901_601.jpg
GTX38674_20190129_WB_19012901_758.jpg
GTX38674_20190129_IHC-P_19012901_494.jpg

Cat No. GTX38674

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Specificity/Sensitivity

HSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G -P-S(p)-W-D).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family B (Small) Member 1,Cmt2F,Hel-S-102,Hmn2B,Hs.76067,Hsp27,Hsp28,Hsp25,Srp27,Hspb1

Cellular Localization

Cytoplasm,Cytoskeleton,Nucleus,Spindle

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
GTX38674_20190129_ICCIF_19012901_601.jpg

GTX38674 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX38674 HSP27 (phospho Ser15) antibody.

GTX38674_20190129_WB_19012901_758.jpg

GTX38674 WB Image

WB analysis of HeLa cell lysate treated or untreated with UV using HSP27 antibody (Lane 1 and 2) and GTX38674 HSP27 (phospho Ser15) antibody (Lane 3 and 4).

GTX38674_20190129_IHC-P_19012901_494.jpg

GTX38674 IHC-P Image

IHC-P analysis of human breast cacinoma tissue using GTX38674 HSP27 (phospho Ser15) antibody.

Package List Price ($)
$ 219