HSP27 (phospho Ser15) antibody

Cat. No. GTX38674

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	50 μl ($399)

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Datasheet
Document

View product citations for antibody GTX38674 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200
IHC-P	1:50-1:100

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

HSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G -P-S(p)-W-D).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Gene ID: 3315 HSPB1
UniProt: P04792 HSPB1

Research Area

DATA IMAGES

GTX38674 WB Image

WB analysis of HeLa cell lysate treated or untreated with UV using HSP27 antibody (Lane 1 and 2) and GTX38674 HSP27 (phospho Ser15) antibody (Lane 3 and 4).

GTX38674 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX38674 HSP27 (phospho Ser15) antibody.

GTX38674 IHC-P Image

IHC-P analysis of human breast cacinoma tissue using GTX38674 HSP27 (phospho Ser15) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty