*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15.
PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G -P-S(p)-W-D).
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Heat Shock Protein Family B (Small) Member 1,Cmt2F,Hel-S-102,Hmn2B,Hs.76067,Hsp27,Hsp28,Hsp25,Srp27,Hspb1
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
GTX38674 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX38674 HSP27 (phospho Ser15) antibody.
GTX38674 WB Image
WB analysis of HeLa cell lysate treated or untreated with UV using HSP27 antibody (Lane 1 and 2) and GTX38674 HSP27 (phospho Ser15) antibody (Lane 3 and 4).
GTX38674 IHC-P Image
IHC-P analysis of human breast cacinoma tissue using GTX38674 HSP27 (phospho Ser15) antibody.