Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser78) antibody [GT1285] (GTX03197) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of HeLa cells were treated by UV at room temperature for 15-30 minutes using GTX03197 HSP27 (phospho Ser78) antibody [GT1285].
Dilution : 1:1000
Loading : 25μg per lane