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HSP27 (phospho Ser82) antibody [GT357]

Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421
Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421
Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421
Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421

Cat No. GTX634421

Host

Mouse

Clonality

Monoclonal

Clone Name

GT357

Isotype

IgG2b

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLa , HeLa (25 μg/ml Anisomycin treatment for 30 min)

Predict Reactivity

Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human HSP27 (phospho Ser82). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888450

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm , Nucleus , Spindle

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421

GTX634421 WB Image

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421

GTX634421 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421

GTX634421 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421

GTX634421 ICC/IF Image

HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

There are currently no references for HSP27 (phospho Ser82) antibody [GT357] (GTX634421). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HSP27 (phospho Ser82) antibody [GT357] (GTX634421). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169