Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (GTX133850) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (GTX133850) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (GTX133850) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.