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HSP27 (phospho Ser82) antibody

Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX17677
Anti-HSP27 (phospho Ser82) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17677
Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX17677
Anti-HSP27 (phospho Ser82) antibody used in ELISA (ELISA). GTX17677

Cat No. GTX17677

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P Assay dependent
ELISA 1:20000-1:40000

Note :

WB
We recommend incubating membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human HSP27 around the phosphorylation site of Serine 82.

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CMT2F , HELS102 , HMN2B , HS.76067 , HSP27 , HSP28 , HSPB1 , Hsp25 , SRP27 , heat shock protein family B (small) member 1

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm,Cytoskeleton,Spindle

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX17677

GTX17677 IHC-P Image

IHC-P analysis of human kidney tissue sections using GTX17677 HSP27 (phospho Ser82) antibody.
Antigen retrieval : Heat mediated antigen retrieval in citrate buffer
Dilution : 1:200

Anti-HSP27 (phospho Ser82) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17677

GTX17677 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX17677 HSP27 (phospho Ser82) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX17677

GTX17677 WB Image

WB analysis of various samples with P (phospho-) and N (non phospho-) peptide using GTX17677 HSP27 (phospho Ser82) antibody.

Anti-HSP27 (phospho Ser82) antibody used in ELISA (ELISA). GTX17677

GTX17677 ELISA Image

ELISA analysis of P (phospho-) and N (non phosho-) immunogen peptide using GTX17677 HSP27 (phospho Ser82) antibody.
Peptides concentration: 1μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339