APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| Assay dependent |
| 1:20000-1:40000 |
|
Note :
WB
We recommend incubating membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human HSP27 around the phosphorylation site of Serine 82.
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CMT2F , HELS102 , HMN2B , HS.76067 , HSP27 , HSP28 , HSPB1 , Hsp25 , SRP27 , heat shock protein family B (small) member 1
Cellular Localization
Cytoplasm,Nucleus,Cytoplasm,Cytoskeleton,Spindle
Background
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX17677 ELISA Image
ELISA analysis of P (phospho-) and N (non phosho-) immunogen peptide using GTX17677 HSP27 (phospho Ser82) antibody.
Peptides concentration: 1μg/ml
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GTX17677 IHC-P Image
IHC-P analysis of human kidney tissue sections using GTX17677 HSP27 (phospho Ser82) antibody.
Antigen retrieval : Heat mediated antigen retrieval in citrate buffer
Dilution : 1:200
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GTX17677 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX17677 HSP27 (phospho Ser82) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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GTX17677 WB Image
WB analysis of various samples with P (phospho-) and N (non phospho-) peptide using GTX17677 HSP27 (phospho Ser82) antibody.
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REFERENCE
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REVIEW
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