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HSP27 (phospho Ser82) antibody

Orthogonal
Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX17937
Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX17937
Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX17937
Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX17937

Cat. No. GTX17937

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
50 μl ($399)
See all HSP27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IHC-P 1:20-1:200
FACS 1:20
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human HSP27 that contains serine 82.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX17937

GTX17937 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX17937 HSP27 (phospho Ser82) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX17937

GTX17937 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX17937 HSP27 (phospho Ser82) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX17937

GTX17937 WB Image

WB analysis of samples using GTX17937 HSP27 (phospho Ser82) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX17937

GTX17937 WB Image

Orthogonal

WB analysis of HeLa (lane1), HeLa exposed for 40 minutes with UV (lane2), K-562 (lane3), K-562 exposed for 40 minutes with UV (lane4) lysate using GTX17937 HSP27 (phospho Ser82) antibody.
Loading : 20 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399