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HSP27 (phospho Ser82) antibody

Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX50277
Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX50277
Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX50277

Cat No. GTX50277

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 82 (Q-L-S(p)-S-G) derived from human HSP27.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX50277

GTX50277 WB Image

WB analysis of extracts from HeLa cells treated with UV or calf intestinal phosphatase (CIP) using GTX50277 HSP27 (phospho Ser82) antibody.

Anti-HSP27 (phospho Ser82) antibody used in Western Blot (WB). GTX50277

GTX50277 WB Image

WB analysis of extracts from HL-60 cells untreated or treated with UV using GTX50277 HSP27 (phospho Ser82) antibody.

Anti-HSP27 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX50277

GTX50277 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50277 HSP27 (phospho Ser82) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf