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HSP47 antibody, C-term

Anti-HSP47 antibody, C-term used in Flow cytometry (FACS). GTX81503
Anti-HSP47 antibody, C-term used in Western Blot (WB). GTX81503
Anti-HSP47 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81503

Cat. No. GTX81503

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all HSP47 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 390-418 amino acids from the C-terminal region of human SERPINH1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family H member 1 , AsTP3 , CBP1 , CBP2 , HSP47 , OI10 , PIG14 , PPROM , RA-A47 , SERPINH2 , gp46

Background

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Database

  • Gene ID: 871 SERPINH1
  • UniProt: P50454 SERPINH1

Research Area

DATA IMAGES

Anti-HSP47 antibody, C-term used in Flow cytometry (FACS). GTX81503

GTX81503 FACS Image

FACS analysis of HepG2 cells using GTX81503 HSP47 antibody, C-term.
Green : primary antibody
Blue : negative control

Anti-HSP47 antibody, C-term used in Western Blot (WB). GTX81503

GTX81503 WB Image

WB analysis of HeLa cell lysate (35ug/lane) using GTX81503 HSP47 antibody, C-term.

Anti-HSP47 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81503

GTX81503 IHC-P Image

IHC-P analysis of human testis tissue using GTX81503 HSP47 antibody, C-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399