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HSP47 antibody [N2C2], Internal

Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

Cat No. GTX103011

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

NIH-3T3 , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Rat, Sheep, Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630639

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family H member 1 , AsTP3 , CBP1 , CBP2 , HSP47 , OI10 , PIG14 , PPROM , RA-A47 , SERPINH2 , gp46

Cellular Localization

Endoplasmic reticulum lumen

Background

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. [provided by RefSeq]

Database

  • Gene ID: 871 SERPINH1
  • UniProt: P50454 SERPINH1

Research Area

DATA IMAGES

Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

GTX103011 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX103011 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011

GTX103011 IHC-P Image

HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

GTX103011 WB Image

HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011

GTX103011 IHC-P Image

HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

GTX103011 WB Image

HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for HSP47 antibody [N2C2], Internal (GTX103011). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HSP47 antibody [N2C2], Internal (GTX103011). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169