APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Rat, Sheep, Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.37 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10630639
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serpin family H member 1 , AsTP3 , CBP1 , CBP2 , HSP47 , OI10 , PIG14 , PPROM , RA-A47 , SERPINH2 , gp46
Cellular Localization
Endoplasmic reticulum lumen
Background
This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. [provided by RefSeq]
Database
-
Gene ID: 871
SERPINH1
-
UniProt: P50454
SERPINH1
Research Area
DATA IMAGES
![Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011](/templates/default/js/lightbox/images/loading.gif "Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011")
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GTX103011 IHC-P Image
HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103011 WB Image
HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103011 WB Image
Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX103011 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103011 IHC-P Image
HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103011 WB Image
HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for HSP47 antibody [N2C2], Internal (GTX103011). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP47 antibody [N2C2], Internal (GTX103011). Be the first to share your experience with this product.
![Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011](/upload/website/prouct_img/small/GTX103011/GTX103011_40150_IHC_w_23060119_320.webp "Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011](/upload/website/prouct_img/small/GTX103011/GTX103011_40150_WB_2_w_23060119_966.webp "Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011](/upload/website/prouct_img/small/GTX103011/GTX103011_40150_WB_M_w_23060119_803.webp "Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011](/upload/website/prouct_img/small/GTX103011/GTX103011_40150_IHC_M_w_23060119_935.webp "Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011](/upload/website/prouct_img/small/GTX103011/GTX103011_40150_WB_w_23060119_770.webp "Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011](/upload/website/prouct_img/normal/GTX103011/GTX103011_40150_IHC_w_23060119_320.webp "Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011](/upload/website/prouct_img/normal/GTX103011/GTX103011_40150_WB_2_w_23060119_966.webp "Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011](/upload/website/prouct_img/normal/GTX103011/GTX103011_40150_WB_M_w_23060119_803.webp "Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011](/upload/website/prouct_img/normal/GTX103011/GTX103011_40150_IHC_M_w_23060119_935.webp "Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011")
![Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011](/upload/website/prouct_img/normal/GTX103011/GTX103011_40150_WB_w_23060119_770.webp "Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011")