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HSP47 antibody [N2C2], Internal

Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011
Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

Cat No. GTX103011

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

NIH-3T3 , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Rat, Sheep, Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

serpin family H member 1 , AsTP3 , CBP1 , CBP2 , HSP47 , OI10 , PIG14 , PPROM , RA-A47 , SERPINH2 , gp46

Cellular Localization

Endoplasmic reticulum lumen

Background

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. [provided by RefSeq]

Database

  • Gene ID: 871 SERPINH1
  • UniProt: P50454 SERPINH1

Research Area

DATA IMAGES
Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

GTX103011 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX103011 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011

GTX103011 IHC-P Image

HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

GTX103011 WB Image

HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 µg U87-MG whole cell lysate/extract
B. 30 µg SK-N-SH whole cell lysate/extract
C. 30 µg IMR32 whole cell lysate/extract
D. 30 µg SK-N-AS whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP47 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103011

GTX103011 IHC-P Image

HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011

GTX103011 WB Image

HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg HepG2 whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169