WB analysis of 50 ug of the indicated whole cell lysates using GTX25479 HSP60 antibody [2E1/53].
Dilution : 1 μg/ml

ICC/IF analysis of NIH-3T3 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

IP analysis of HeLa cell lysates using GTX25479 HSP60 antibody [2E1/53].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1 μg/ml

IHC-P analysis of human kidney tissue using GTX25479 HSP60 antibody [2E1/53].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

ICC/IF analysis of HeLa cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

IHC-P analysis of human breast carcinoma tissue using GTX25479 HSP60 antibody [2E1/53].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

ICC/IF analysis of HeLa cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of NIH-3T3 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25479 HSP60 antibody [2E1/53].
Green : Primary antibody
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 10 μg/ml for at least 1 hour at room temperature

ICC/IF analysis of U251 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of A431 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight in a humidified chamber