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HSP60 antibody [2E1/53]

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479
Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479
Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479
Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479
Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479
Anti-HSP60 antibody [2E1/53] used in IHC (Paraffin sections) (IHC-P). GTX25479
Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479
Anti-HSP60 antibody [2E1/53] used in IHC (Paraffin sections) (IHC-P). GTX25479
Anti-HSP60 antibody [2E1/53] used in Immunoprecipitation (IP). GTX25479
Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479
Anti-HSP60 antibody [2E1/53] used in Western Blot (WB). GTX25479

Cat. No. GTX25479

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2E1/53

Isotype

 IgM

Application

 WB, ICC/IF, IHC-P, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μg ($399)
See all HSP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-10 μg/ml
ICC/IF 1:20-1:200
IHC-P 1-10 μg/ml
IP 2 μg
ELISA 1-10 μg/ml
IHC Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds between amino acids 211-288.

PROPERTIES

Form

Liquid

Buffer

buffer, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human placental HSP60.

Purification

Purified IgM

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479

GTX25479 ICC/IF Image

ICC/IF analysis of A431 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight in a humidified chamber

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479

GTX25479 ICC/IF Image

ICC/IF analysis of U251 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479

GTX25479 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25479 HSP60 antibody [2E1/53].
Green : Primary antibody
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 10 μg/ml for at least 1 hour at room temperature

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479

GTX25479 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479

GTX25479 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-HSP60 antibody [2E1/53] used in IHC (Paraffin sections) (IHC-P). GTX25479

GTX25479 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX25479 HSP60 antibody [2E1/53].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479

GTX25479 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

Anti-HSP60 antibody [2E1/53] used in IHC (Paraffin sections) (IHC-P). GTX25479

GTX25479 IHC-P Image

IHC-P analysis of human kidney tissue using GTX25479 HSP60 antibody [2E1/53].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-HSP60 antibody [2E1/53] used in Immunoprecipitation (IP). GTX25479

GTX25479 IP Image

IP analysis of HeLa cell lysates using GTX25479 HSP60 antibody [2E1/53].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1 μg/ml

Anti-HSP60 antibody [2E1/53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25479

GTX25479 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-HSP60 antibody [2E1/53] used in Western Blot (WB). GTX25479

GTX25479 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX25479 HSP60 antibody [2E1/53].
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399