GeneTex
  • Country / Location Selection

United States (US)

HSP60 antibody [2E4]

Anti-HSP60 antibody [2E4] used in Western Blot (WB). GTX50050
Anti-HSP60 antibody [2E4] used in IHC (Paraffin sections) (IHC-P). GTX50050

Cat No. GTX50050

Host

Mouse

Clonality

Monoclonal

Clone Name

2E4

Isotype

IgG1

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot is 1:1,000 ~ 1:2,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Hsp60 (1-573aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Heat Shock Protein Family D (Hsp60) Member 1 , Cpn60 , Groel , Hld4 , Hsp-60 , Hsp60 , Hsp65 , Hucha60 , Spg13 , Hspd1

Background

Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [2E4] used in Western Blot (WB). GTX50050

GTX50050 WB Image

Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp60 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-HSP60 antibody [2E4] used in IHC (Paraffin sections) (IHC-P). GTX50050

GTX50050 IHC-P Image

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

REFERENCE

There are currently no references for HSP60 antibody [2E4] (GTX50050). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HSP60 antibody [2E4] (GTX50050). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf