APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human HSP60 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13
Cellular Localization
Mitochondrion matrix
Background
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
Database
Research Area
DATA IMAGES
![Anti-HSP60 antibody [3G8] used in ELISA (ELISA). GTX60424](/templates/default/js/lightbox/images/loading.gif "Anti-HSP60 antibody [3G8] used in ELISA (ELISA). GTX60424")
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GTX60424 ELISA Image
ELISA analysis of antigen using GTX60424 HSP60 antibody [3G8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60424 WB Image
WB analysis of T47D (1), HeLa (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60424 HSP60 antibody [3G8].
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GTX60424 FACS Image
FACS analysis of HeLa cells using GTX60424 HSP60 antibody [3G8].
Green : HSP60
Purple : negative control
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GTX60424 ICC/IF Image
ICC/IF analysis of 3T3-L1 cells using GTX60424 HSP60 antibody [3G8].
Green : HSP60
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60424 IHC-P Image
IHC-P analysis of breast cancer tissue (left) and colon cancer tissue (right) using GTX60424 HSP60 antibody [3G8].
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GTX60424 IHC-P Image
IHC-P analysis of lung cancer tissue (left) and kidney cancer tissue (right) using GTX60424 HSP60 antibody [3G8].
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REFERENCE
There are currently no references for HSP60 antibody [3G8] (GTX60424). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP60 antibody [3G8] (GTX60424). Be the first to share your experience with this product.
![Anti-HSP60 antibody [3G8] used in ELISA (ELISA). GTX60424](/upload/website/prouct_img/small/GTX60424/GTX60424_20170912_ELISA_w_23061123_845.webp "Anti-HSP60 antibody [3G8] used in ELISA (ELISA). GTX60424")
![Anti-HSP60 antibody [3G8] used in Western Blot (WB). GTX60424](/upload/website/prouct_img/small/GTX60424/GTX60424_20170912_WB_w_23061123_843.webp "Anti-HSP60 antibody [3G8] used in Western Blot (WB). GTX60424")
![Anti-HSP60 antibody [3G8] used in Flow cytometry (FACS). GTX60424](/upload/website/prouct_img/small/GTX60424/GTX60424_20170912_FACS_w_23061123_352.webp "Anti-HSP60 antibody [3G8] used in Flow cytometry (FACS). GTX60424")
![Anti-HSP60 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60424](/upload/website/prouct_img/small/GTX60424/GTX60424_20170912_ICCIF_w_23061123_848.webp "Anti-HSP60 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60424")
![Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424](/upload/website/prouct_img/small/GTX60424/GTX60424_20170912_IHC-P_w_23061123_754.webp "Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424")
![Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424](/upload/website/prouct_img/small/GTX60424/GTX60424_20170912_IHC-P_1_w_23061123_246.webp "Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424")
![Anti-HSP60 antibody [3G8] used in ELISA (ELISA). GTX60424](/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_ELISA_w_23061123_845.webp "Anti-HSP60 antibody [3G8] used in ELISA (ELISA). GTX60424")
![Anti-HSP60 antibody [3G8] used in Western Blot (WB). GTX60424](/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_WB_w_23061123_843.webp "Anti-HSP60 antibody [3G8] used in Western Blot (WB). GTX60424")
![Anti-HSP60 antibody [3G8] used in Flow cytometry (FACS). GTX60424](/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_FACS_w_23061123_352.webp "Anti-HSP60 antibody [3G8] used in Flow cytometry (FACS). GTX60424")
![Anti-HSP60 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60424](/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_ICCIF_w_23061123_848.webp "Anti-HSP60 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60424")
![Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424](/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_IHC-P_w_23061123_754.webp "Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424")
![Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424](/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_IHC-P_1_w_23061123_246.webp "Anti-HSP60 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX60424")