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HSP60 antibody [4B9/89]

Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478
Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478
Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478
Anti-HSP60 antibody [4B9/89] used in Flow cytometry (FACS). GTX25478
Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478
Anti-HSP60 antibody [4B9/89] used in IHC (Paraffin sections) (IHC-P). GTX25478
Anti-HSP60 antibody [4B9/89] used in IHC (Paraffin sections) (IHC-P). GTX25478
Anti-HSP60 antibody [4B9/89] used in Western Blot (WB). GTX25478
Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478
Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478
Anti-HSP60 antibody [4B9/89] used in Immunoprecipitation (IP). GTX25478
Anti-HSP60 antibody [4B9/89] used in IHC (Paraffin sections) (IHC-P). GTX25478

Cat. No. GTX25478

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B9/89

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Bacteria, Primate
Package
100 μg ($399)
See all HSP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:1000
ICC/IF 10 μg/ml
IHC-P 1:200
FACS 1-20 μg/ml
IP 2μg
ELISA Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds either between amino acids 335-366 or 484-547.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human placental HSP60.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_373944

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478

GTX25478 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478

GTX25478 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25478 HSP60 antibody [4B9/89].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478

GTX25478 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-HSP60 antibody [4B9/89] used in Flow cytometry (FACS). GTX25478

GTX25478 FACS Image

FACS analysis of permeabilized, fixed HeLa cells using GTX25478 HSP60 antibody [4B9/89].
Orange histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 μg/ml incubated for 1 hour on ice

Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478

GTX25478 ICC/IF Image

ICC/IF analysis of human endothelial cells using GTX25478 HSP60 antibody [4B9/89].

Anti-HSP60 antibody [4B9/89] used in IHC (Paraffin sections) (IHC-P). GTX25478

GTX25478 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

Anti-HSP60 antibody [4B9/89] used in IHC (Paraffin sections) (IHC-P). GTX25478

GTX25478 IHC-P Image

IHC-P analysis of human kidney tissue using GTX25478 HSP60 antibody [4B9/89].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-HSP60 antibody [4B9/89] used in Western Blot (WB). GTX25478

GTX25478 WB Image

WB analysis of 50μg of the indicated whole cell lysates using GTX25478 HSP60 antibody [4B9/89].
Dilution : 1:1000

Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478

GTX25478 ICC/IF Image

ICC/IF analysis of ATDC5 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-HSP60 antibody [4B9/89] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25478

GTX25478 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-HSP60 antibody [4B9/89] used in Immunoprecipitation (IP). GTX25478

GTX25478 IP Image

IP analysis of HeLa cell lysates using GTX25478 HSP60 antibody [4B9/89].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

Anti-HSP60 antibody [4B9/89] used in IHC (Paraffin sections) (IHC-P). GTX25478

GTX25478 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25478 HSP60 antibody [4B9/89].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

REFERENCE

Application Reference

Bult? DB et al. Biotechnol Bioeng 2020; 117 (9) : 2633-2647 Overexpression of the mitochondrial pyruvate carrier reduces lactate production and increases recombinant protein productivity in CHO cells.
Application : WB
Lodato P.B. et al. FEMS Microbiol Lett 2017; (Epub) Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7.
Application : WB

Clone Reference

Anke Osterloh et al. J Biol Chem . 2007; 282 (7):4669-4680 Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide
Application : Neutralizing/Inhibition
Reactivity : Mouse
Submit a Reference

REVIEW

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Package List Price ($)
$ 399