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HSP60 antibody [GROEL/730]

Anti-HSP60 antibody [GROEL/730] used in Western Blot (WB). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787
Anti-HSP60 antibody [GROEL/730] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

Cat No. GTX34787

Host

Mouse

Clonality

Monoclonal

Clone Name

GROEL/730

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA buffer, pH 8.5-9.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Specificity/Sensitivity

Clone GROEL730 reacts only with human and is useful in distinguishing human hsp60 from other mammalian and bacterial hsp60.
We do not recommend use of this product for Mouse,Rat.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HSP60 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CPN60 , GROEL , HLD4 , HSP60 , HSP65 , HSPD1 , HuCHA60 , SPG13 , heat shock protein family D (Hsp60) member 1 , Heat Shock Protein 60

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [GROEL/730] used in Western Blot (WB). GTX34787

GTX34787 WB Image

WB analysis of HeLa cell lysate using GTX34787 HSP60 antibody [GROEL/730].

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34787 HSP60 antibody [GROEL/730].

Anti-HSP60 antibody [GROEL/730] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34787

GTX34787 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34787 HSP60 antibody [GROEL/730].
Green : Primary antibody
Red : nucleus

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX34787 HSP60 antibody [GROEL/730].

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

IHC-P analysis of human testicular carcinoma tissue using GTX34787 HSP60 antibody [GROEL/730].

REFERENCE

There are currently no references for HSP60 antibody [GROEL/730] (GTX34787). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HSP60 antibody [GROEL/730] (GTX34787). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf