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HSP60 antibody [GROEL/730]

Anti-HSP60 antibody [GROEL/730] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34787
Anti-HSP60 antibody [GROEL/730] used in Western Blot (WB). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

Cat. No. GTX34787

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GROEL/730

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all HSP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA buffer, pH 8.5-9.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

Clone GROEL730 reacts only with human and is useful in distinguishing human hsp60 from other mammalian and bacterial hsp60.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HSP60 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CPN60 , GROEL , HLD4 , HSP60 , HSP65 , HSPD1 , HuCHA60 , SPG13 , heat shock protein family D (Hsp60) member 1 , Heat Shock Protein 60

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [GROEL/730] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34787

GTX34787 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34787 HSP60 antibody [GROEL/730].
Green : Primary antibody
Red : nucleus

Anti-HSP60 antibody [GROEL/730] used in Western Blot (WB). GTX34787

GTX34787 WB Image

WB analysis of HeLa cell lysate using GTX34787 HSP60 antibody [GROEL/730].

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX34787 HSP60 antibody [GROEL/730].

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

IHC-P analysis of human testicular carcinoma tissue using GTX34787 HSP60 antibody [GROEL/730].

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34787 HSP60 antibody [GROEL/730].

REFERENCE

Application Reference

De Maio F et al. Virulence 2021; 12 (1) : 868-884 PE_PGRS3 ensures provision of the vital phospholipids cardiolipin and phosphatidylinositols by promoting the interaction between M. tuberculosis and host cells.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529