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HSP60 antibody [GROEL/730]

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

Cat No. GTX34787

Host Mouse
Clonality Monoclonal
Clone Name GROEL/730
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srp?s). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone GROEL730 reacts only with human and is useful in distinguishing human hsp60 from other mammalian and bacterial hsp60.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human HSP60 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family D (Hsp60) Member 1 , Cpn60 , Groel , Hld4 , Hsp-60 , Hsp60 , Hsp65 , Hucha60 , Spg13 , Hspd1

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES
Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with HSP60 Monoclonal Antibody (GROEL/730)

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with HSP60 Monoclonal Antibody (GROEL/730)

Anti-HSP60 antibody [GROEL/730] used in IHC (Paraffin sections) (IHC-P). GTX34787

GTX34787 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreas stained with HSP60 Monoclonal Antibody (GROEL/730).

REFERENCE
There are currently no references for HSP60 antibody [GROEL/730] (GTX34787). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 429