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HSP60 antibody [HSPD1/2206R]

Anti-HSP60 antibody [HSPD1/2206R] used in Western Blot (WB). GTX02649
Anti-HSP60 antibody [HSPD1/2206R] used in IHC (Paraffin sections) (IHC-P). GTX02649

Cat. No. GTX02649

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HSPD1/2206R

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Chicken, Pig, Monkey
Package
100 μg ($529)
See all HSP60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/ml
IHC-P 1-2 μg/ml

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length HSP60 protein. An epitope is localized between aa 383-447 of human HSP60.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family D (Hsp60) member 1 , CPN60 , GROEL , HLD4 , HSP-60 , HSP60 , HSP65 , HuCHA60 , SPG13

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [HSPD1/2206R] used in Western Blot (WB). GTX02649

GTX02649 WB Image

WB analysis of HeLa whole cell lysate using GTX02649 HSP60 antibody [HSPD1/2206R].

Anti-HSP60 antibody [HSPD1/2206R] used in IHC (Paraffin sections) (IHC-P). GTX02649

GTX02649 IHC-P Image

IHC-P analysis of human liver tissue section using GTX02649 HSP60 antibody [HSPD1/2206R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529