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HSP60 antibody [HSPD1/780]

Anti-HSP60 antibody [HSPD1/780] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34788
Anti-HSP60 antibody [HSPD1/780] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34788
Anti-HSP60 antibody [HSPD1/780] used in Western Blot (WB). GTX34788
Anti-HSP60 antibody [HSPD1/780] used in Protein Array (Protein Array). GTX34788
Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788
Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788
Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788
Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788

Cat No. GTX34788

Host

Mouse

Clonality

Monoclonal

Clone Name

HSPD1/780

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, Protein Array

Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Chicken, Pig, Monkey
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HSPD1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CPN60 , GROEL , HLD4 , HSP60 , HSP65 , HSPD1 , HuCHA60 , SPG13 , heat shock protein family D (Hsp60) member 1 , Heat Shock Protein 60

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-HSP60 antibody [HSPD1/780] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34788

GTX34788 ICC/IF Image

ICC/IF analysis of MeOH-fixed HeLa cells using GTX34788 HSP60 antibody [HSPD1/780].
Green : Primary antibody
Red : nucleus

Anti-HSP60 antibody [HSPD1/780] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34788

GTX34788 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34788 HSP60 antibody [HSPD1/780].
Green : Primary antibody
Red : nucleus

Anti-HSP60 antibody [HSPD1/780] used in Western Blot (WB). GTX34788

GTX34788 WB Image

WB analysis of HeLa cell lysate using GTX34788 HSP60 antibody [HSPD1/780].

Anti-HSP60 antibody [HSPD1/780] used in Protein Array (Protein Array). GTX34788

GTX34788 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP60-Monospecific Mouse Monoclonal Antibody (HSPD1/780). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788

GTX34788 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX34788 HSP60 antibody [HSPD1/780].

Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788

GTX34788 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX34788 HSP60 antibody [HSPD1/780].

Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788

GTX34788 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX34788 HSP60 antibody [HSPD1/780].

Anti-HSP60 antibody [HSPD1/780] used in IHC (Paraffin sections) (IHC-P). GTX34788

GTX34788 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX34788 HSP60 antibody [HSPD1/780].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf