APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.25-0.5μg/ml |
1-2μg/ml |
1-2μg/ml for 30 minutes at RT |
1-2μg/10⁶ cells |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
Epitope is localized between aa 383-447 of human hsp60.
We do not recommend use of this product for Spinach,Bacteria,Helminths samples.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human HSP60 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CPN60 , GROEL , HLD4 , HSP60 , HSP65 , HSPD1 , HuCHA60 , SPG13 , heat shock protein family D (Hsp60) member 1 , Heat Shock Protein 60
Cellular Localization
Mitochondrion matrix
Background
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
Database
Research Area
DATA IMAGES
|
GTX34785 ICC/IF Image
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34785 HSP60 antibody [LK1]. Green : Primary antibody Red : nucleus
|
|
GTX34785 FACS Image
FACS analysis of paraformaldehyde-fixed HeLa cells using GTX34785 HSP60 antibody [LK1]. Blue : Primary antibody Red : Isotype control
|
|
GTX34785 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX34785 HSP60 antibody [LK1].
|
|
GTX34785 WB Image
WB analysis of HeLa cell lysate using GTX34785 HSP60 antibody [LK1].
|
|
GTX34785 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX34785 HSP60 antibody [LK1].
|
REFERENCE
There are currently no references for HSP60 antibody [LK1] (GTX34785). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP60 antibody [LK1] (GTX34785). Be the first to share your experience with this product.